diff --git a/bsp/stm32/stm32f407-rt-spark/applications/arduino_main.cpp b/bsp/stm32/stm32f407-rt-spark/applications/arduino_main.cpp
new file mode 100644
index 0000000000..1335fec08c
--- /dev/null
+++ b/bsp/stm32/stm32f407-rt-spark/applications/arduino_main.cpp
@@ -0,0 +1,24 @@
+/*
+ * Copyright (c) 2006-2024, RT-Thread Development Team
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ *
+ * Change Logs:
+ * Date           Author         Notes
+ * 2024-04-08     Li ZhenHong    first version
+ */
+
+#include <Arduino.h>
+
+void setup(void)
+{
+    /* put your setup code here, to run once: */
+    Serial.begin();
+}
+
+void loop(void)
+{
+    /* put your main code here, to run repeatedly: */
+    Serial.println("Hello Arduino!");
+    delay(800);
+}
diff --git a/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/README.md b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/README.md
new file mode 100644
index 0000000000..330a1089ff
--- /dev/null
+++ b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/README.md
@@ -0,0 +1,69 @@
+# stm32f407-rt-spark 开发板的Arduino生态兼容说明
+
+## 1 RTduino - RT-Thread的Arduino生态兼容层
+
+stm32f407-rt-spark 开发板已经完整适配了[RTduino软件包](https://github.com/RTduino/RTduino)，即RT-Thread的Arduino生态兼容层。用户可以按照Arduino的编程习惯来操作该BSP，并且可以使用大量Arduino社区丰富的库，是对RT-Thread生态的极大增强。更多信息，请参见[RTduino软件包说明文档](https://github.com/RTduino/RTduino)。
+
+### 1.1 如何开启针对本BSP的Arduino生态兼容层
+
+Env 工具下敲入 menuconfig 命令，或者 RT-Thread Studio IDE 下选择 RT-Thread Settings：
+
+```Kconfig
+Hardware Drivers Config --->
+    Onboard Peripheral Drivers --->
+        [*] Compatible with Arduino Ecosystem (RTduino)
+```
+
+## 2 Arduino引脚排布
+
+更多引脚布局相关信息参见 [pins_arduino.c](pins_arduino.c) 和 [pins_arduino.h](pins_arduino.h)。
+
+![Rt-spark_Rtduino_Pin_Map.drawio](Rt-spark_Rtduino_Pin_Map.drawio.png)
+
+| Arduino引脚编号  | STM32引脚编号 | 5V容忍 | 备注  |
+| ------------------- | --------- | ---- | ------------------------------------------------------------------------- |
+| 0 (D0) | GET_PIN(A, 10) | 是 | Serial-RX,默认被RT-Thread的UART设备框架uart1接管 |
+| 1 (D1) | GET_PIN(A, 9) | 是 | Serial-TX,默认被RT-Thread的UART设备框架uart1接管 |
+| 2 (D2) | GET_PIN(A, 8) | 是 |  |
+| 3 (D3) | GET_PIN(B, 10) | 是 | PWM2-CH3,默认被RT-Thread的PWM设备框架pwm2接管 |
+| 4 (D4) | GET_PIN(A, 1) | 是 |  |
+| 5 (D5) | GET_PIN(B, 14) | 是 |  |
+| 6 (D6) | GET_PIN(B, 11) | 是 | PWM2-CH4,默认被RT-Thread的PWM设备框架pwm2接管 |
+| 7 (D7) | GET_PIN(B, 15) | 是 |  |
+| 8 (D8) | GET_PIN(F, 15) | 是 |  |
+| 9 (D9) | GET_PIN(E, 11) | 是 | PWM1-CH2,默认被RT-Thread的PWM设备框架pwm1接管 |
+| 10 (D10) | GET_PIN(E, 13) | 是 | PWM1-CH3,默认被RT-Thread的PWM设备框架pwm1接管 |
+| 11 (D11) | GET_PIN(D, 12) | 是 | PWM4-CH1,默认被RT-Thread的PWM设备框架pwm4接管 |
+| 12 (D12) | GET_PIN(D, 10) | 是 |  |
+| 13 (D13) | GET_PIN(D, 8) | 是 |  |
+| 14 (D14) | GET_PIN(E, 14) | 是 |  |
+| 15 (D15) | GET_PIN(E, 12) | 是 |  |
+| 16 (D16) | GET_PIN(E, 15) | 是 |  |
+| 17 (D17) | GET_PIN(D, 9) | 是 |  |
+| 18 (D18) | GET_PIN(G, 2) | 是 |  |
+| 19 (D19) | GET_PIN(B, 2) | 是 |  |
+| 20 (D20) | GET_PIN(G, 0) | 是 |  |
+| 21 (D21) | GET_PIN(A, 0) | 是 |  |
+| 22 (D22) | GET_PIN(G, 5) | 是 | SSPI1-SCK,默认被RT-Thread的SPI设备框架sspi1接管|
+| 23 (D23) | GET_PIN(G, 3) | 是 | SSPI1-MISO,默认被RT-Thread的SPI设备框架sspi1接管 |
+| 24 (D24) | GET_PIN(G, 1) | 是 | SSPI1-MOSI,默认被RT-Thread的SPI设备框架sspi1接管 |
+| 25 (D25) | GET_PIN(G, 7) | 是 | I2C1-SCL,默认被RT-Thread的I2C设备框架i2c1接管 |
+| 26 (D26) | GET_PIN(D, 7) | 是 | I2C1-SDA,默认被RT-Thread的I2C设备框架i2c1接管 |
+| 27 (D27) | GET_PIN(B, 6) | 是 | I2C2-SCL,默认被RT-Thread的I2C设备框架i2c2接管 |
+| 28 (D28) | GET_PIN(B, 7) | 是 | I2C2-SDA,默认被RT-Thread的I2C设备框架i2c2接管 |
+| 29 (D29) | GET_PIN(G, 6) | 是 |  |
+| 30 (D30) | GET_PIN(G, 4) | 是 |  |
+| 31 (D31) | GET_PIN(A, 2) | 是 | Serial2-TX,默认被RT-Thread的UART设备框架uart2接管 |
+| 32 (D32) | GET_PIN(A, 3) | 是 | Serial2-RX,默认被RT-Thread的UART设备框架uart2接管 |
+| 33 (D33) | GET_PIN(F, 12) | 是 | 板载用户R_LED |
+| 34 (D34) | GET_PIN(F, 11) | 是 | 板载用户B_LED |
+| 35 (D35) | GET_PIN(B, 0) | 是 | 板载蜂鸣器 |
+| 36 (D36) | GET_PIN(C, 5) | 是 | 板载按键KEY_UP |
+| 37 (D37) | GET_PIN(C, 1) | 是 | 板载按键KEY_DOWM |
+| 38 (D38) | GET_PIN(C, 0) | 是 | 板载按键KEY_LEFT |
+| 39 (D39) | GET_PIN(C, 4) | 是 | 板载按键KEY_RIGHT |
+| 40 (A0) | GET_PIN(F, 6) | 是 | ADC3-CH4,默认被RT-Thread的ADC设备框架adc3接管 |
+| 41 (A1) | GET_PIN(F, 7) | 是 | ADC3-CH5,默认被RT-Thread的ADC设备框架adc3接管 |
+| 42 (A2) | GET_PIN(F, 4) | 是 | ADC3-CH14,默认被RT-Thread的ADC设备框架adc3接管 |
+| 43 (A3) | GET_PIN(F, 5) | 是 | ADC3-CH15,默认被RT-Thread的ADC设备框架adc3接管 |
+| 44 (DAC0) | GET_PIN(A, 4) | 否 | DAC1-CH1,默认被RT-Thread的DAC设备框架dac1接管 |
diff --git a/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/Rt-spark_Rtduino_Pin_Map.drawio.png b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/Rt-spark_Rtduino_Pin_Map.drawio.png
new file mode 100644
index 0000000000..b84867a536
Binary files /dev/null and b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/Rt-spark_Rtduino_Pin_Map.drawio.png differ
diff --git a/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/SConscript b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/SConscript
new file mode 100644
index 0000000000..da85e053c3
--- /dev/null
+++ b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/SConscript
@@ -0,0 +1,9 @@
+from building import *
+
+cwd = GetCurrentDir()
+src = Glob('*.c') + Glob('*.cpp')
+inc = [cwd]
+
+group = DefineGroup('RTduino-pinout', src, depend = ['PKG_USING_RTDUINO'], CPPPATH = inc)
+
+Return('group')
diff --git a/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/pins_arduino.c b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/pins_arduino.c
new file mode 100644
index 0000000000..88fed11beb
--- /dev/null
+++ b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/pins_arduino.c
@@ -0,0 +1,69 @@
+/*
+ * Copyright (c) 2006-2024, RT-Thread Development Team
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ *
+ * Change Logs:
+ * Date           Author         Notes
+ * 2024-04-10     Li ZhenHong    first version
+ */
+
+#include <Arduino.h>
+#include "pins_arduino.h"
+#include <drv_gpio.h>
+
+/*
+ * {Arduino Pin, RT-Thread Pin [, Device Name, Channel]}
+ * [] means optional
+ * Digital pins must NOT give the device name and channel.
+ * Analog pins MUST give the device name and channel(ADC, PWM or DAC).
+ * Arduino Pin must keep in sequence.
+ */
+const pin_map_t pin_map_table[]=
+{
+    {D0, GET_PIN(A, 10), "uart1"},          /* Serial-RX */
+    {D1, GET_PIN(A, 9), "uart1"},           /* Serial-TX */
+    {D2, GET_PIN(A, 8)},
+    {D3, GET_PIN(B, 10), "pwm2", 3},        /* PWM */
+    {D4, GET_PIN(A, 1)},
+    {D5, GET_PIN(B, 14)},
+    {D6, GET_PIN(B, 11), "pwm2", 4},        /* PWM */
+    {D7, GET_PIN(B, 15)},
+    {D8, GET_PIN(F, 15)},
+    {D9, GET_PIN(E, 11), "pwm1", 2},        /* PWM */
+    {D10, GET_PIN(E, 13), "pwm1", 3},       /* PWM */
+    {D11, GET_PIN(D, 12), "pwm4", 1},       /* PWM */
+    {D12, GET_PIN(D, 10)},
+    {D13, GET_PIN(D, 8)},
+    {D14, GET_PIN(E, 14)},
+    {D15, GET_PIN(E, 12)},
+    {D16, GET_PIN(E, 15)},
+    {D17, GET_PIN(D, 9)},
+    {D18, GET_PIN(G, 2)},
+    {D19, GET_PIN(B, 2)},
+    {D20, GET_PIN(G, 0)},
+    {D21, GET_PIN(A, 0)},
+    {D22, GET_PIN(G, 5), "sspi1"},          /* SOFT-SPI-SCK */
+    {D23, GET_PIN(G, 3), "sspi1"},          /* SOFT-SPI-SCK */
+    {D24, GET_PIN(G, 1), "sspi1"},          /* SOFT-SPI-SCK */
+    {D25, GET_PIN(G, 7), "i2c4"},           /* I2C-SCL (Wire) */
+    {D26, GET_PIN(D, 7), "i2c4"},           /* I2C-SDA (Wire) */
+    {D27, GET_PIN(B, 6), "i2c5"},           /* I2C-SCL (Wire) */
+    {D28, GET_PIN(B, 7), "i2c5"},           /* I2C-SDA (Wire) */
+    {D29, GET_PIN(G, 6)},                   /* SPI-SS */
+    {D30, GET_PIN(G, 4)},
+    {D31, GET_PIN(A, 2), "uart2"},          /* Serial2-TX */
+    {D32, GET_PIN(A, 3), "uart2"},          /* Serial2-RX */
+    {D33, GET_PIN(F, 12)},                  /* On-Board R_LED */
+    {D34, GET_PIN(F, 11)},                  /* On-Board B_LED */
+    {D35, GET_PIN(B, 0)},                   /* On_Board Buzzer */
+    {D36, GET_PIN(C, 5)},                   /* On-Board KEY_UP */
+    {D37, GET_PIN(C, 1)},                   /* On-Board KEY_DOWM */
+    {D38, GET_PIN(C, 0)},                   /* On-Board KEY_LEFT */
+    {D39, GET_PIN(C, 4)},                   /* On-Board KEY_RIGHT */
+    {A0, GET_PIN(F, 6), "adc3", 4},         /* ADC */
+    {A1, GET_PIN(F, 7), "adc3", 5},         /* ADC */
+    {A2, GET_PIN(F, 4), "adc3", 14},        /* ADC */
+    {A3, GET_PIN(F, 5), "adc3", 15},        /* ADC */
+    {DAC0, GET_PIN(A, 4), "dac1", 1},       /* DAC */
+};
diff --git a/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/pins_arduino.h b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/pins_arduino.h
new file mode 100644
index 0000000000..12b7d60885
--- /dev/null
+++ b/bsp/stm32/stm32f407-rt-spark/applications/arduino_pinout/pins_arduino.h
@@ -0,0 +1,77 @@
+/*
+ * Copyright (c) 2006-2024, RT-Thread Development Team
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ *
+ * Change Logs:
+ * Date           Author         Notes
+ * 2024-04-07     Li ZhenHong    first version
+ */
+
+#ifndef Pins_Arduino_h
+#define Pins_Arduino_h
+
+/* pins alias. Must keep in sequence */
+#define D0        (0)
+#define D1        (1)
+#define D2        (2)
+#define D3        (3)
+#define D4        (4)
+#define D5        (5)
+#define D6        (6)
+#define D7        (7)
+#define D8        (8)
+#define D9        (9)
+#define D10       (10)
+#define D11       (11)
+#define D12       (12)
+#define D13       (13)
+#define D14       (14)
+#define D15       (15)
+#define D16       (16)
+#define D17       (17)
+#define D18       (18)
+#define D19       (19)
+#define D20       (20)
+#define D21       (21)
+#define D22       (22)
+#define D23       (23)
+#define D24       (24)
+#define D25       (25)
+#define D26       (26)
+#define D27       (27)
+#define D28       (28)
+#define D29       (29)
+#define D30       (30)
+#define D31       (31)
+#define D32       (32)
+#define D33       (33)
+#define D34       (34)
+#define D35       (35)
+#define D36       (36)
+#define D37       (37)
+#define D38       (38)
+#define D39       (39)
+#define A0        (40)
+#define A1        (41)
+#define A2        (42)
+#define A3        (43)
+#define DAC0      (44)
+
+#define RTDUINO_PIN_MAX_LIMIT DAC0 /* pin number max limit check */
+
+#define F_CPU 168000000L /* CPU:168MHz */
+
+#define LED_BUILTIN D33 /* Default Built-in LED */
+
+/* i2c4 : PD.7-SDA PG.7-SCL */
+#define RTDUINO_DEFAULT_IIC_BUS_NAME "i2c4"
+
+#define SS D32 /* Chip select pin of default spi */
+/* sspi1 : PG.5-SCK  PG.3-MISO  PG.1-MOSI */
+#define RTDUINO_DEFAULT_SPI_BUS_NAME "sspi1"
+
+/* Serial2(uart2) : PA.2-TX  PA.3-RX */
+#define RTDUINO_SERIAL2_DEVICE_NAME "uart2"
+
+#endif /* Pins_Arduino_h */